November 1, 2011 by staff
Unexpected Antioxidants, Background: Lutein is an important nutrient for eye protection. This study investigated the protective effects of lutein and mechanisms of lipopolysaccharide (LPS)-induced uveitis in mice.
Methods of lutein, suspended in water to a final concentration of 12.5 and 25mg/mL, was administered to mice in 0.1mL/10g body weight during five consecutive days. Control group received drinking water only models.
Uveitis was induced by LPS injection (100 mg per mouse) in the leg in the model and lutein groups on day 5 after the last drug administration. The eyes of mice were collected 24 hours after LPS injection for the detection of indicators using commercial kits and reverse transcription chain reaction of polymerase.
Results LPS induced uveitis was confirmed by significant pathological damage and increased the level of nitric oxide in ocular tissue of Balb / c 24 hours after injection of the footpad. The elevated level of nitric oxide was significantly reduced by oral administration of lutein (125 and 500mg/kg/d for five days) before injection of LPS.
On the other hand, lutein decreased malondialdehyde content, increased oxygen radical absorption capacity level, glutathione, vitamin C and total superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities. Lutein further increased the expression of the copper-zinc SOD, manganese SOD and GPx mRNA.
Conclusion The antioxidant properties of lutein contribute to the protection against LPS-induced uveitis in part thanks to the intervention of inflammation.
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